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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has been observed in several tumors, which leads to chromosome instability, and so may contribute to tumorigenesis. This protein was uncovered to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a critical role in advertising mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually explained. Two more splice variants have been noted but comprehensive nucleotide sequence data just isn't nonetheless offered.

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Blocking peptides are peptides that bind specifically for the focus on antibody and block antibody binding. These peptide generally includes the epitope identified via the antibody.

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This information outlines further more possible optimization of cellular and immunoassay parameters to make sure the absolute best benefits are...

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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible effects are...

Antibodies certain to the blocking peptide now not bind on the epitope around the concentrate on protein. By comparing the staining with the blocked antibody compared to the antibody by yourself, you can see which staining is distinct.

Target Data Cyclin E belongs for the remarkably conserved cyclin loved ones, whose customers are characterised by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which lead for the temporal coordination of every mitotic occasion. Cyclin E forms a fancy with and features being a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S transition. This protein accumulates within the G1-S section boundary and is particularly degraded as cells development by S period.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...

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